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. 2009 Dec;49(12):2594-8.
doi: 10.1111/j.1537-2995.2009.02344.x. Epub 2009 Aug 4.

Bacterial contamination of pediatric whole blood transfusions in a Kenyan hospital

Oliver Hassall  1 , Kathryn Maitland, Lewa Pole, Salim Mwarumba, Douglas Denje, Kongo Wambua, Brett Lowe, Christopher Parry, Kishor Mandaliya, Imelda Bates
Affiliations
Free PMC article

Bacterial contamination of pediatric whole blood transfusions in a Kenyan hospital

Oliver Hassall et al. Transfusion. 2009 Dec.
Free PMC article

Abstract

Background: Hospitalized children in sub-Saharan Africa frequently receive whole blood transfusions for severe anemia. The risk from bacterial contamination of blood for transfusion in sub-Saharan Africa is not known. This study assessed the frequency of bacterial contamination of pediatric whole blood transfusions at a referral hospital in Kenya.

Study design and methods: This was an observational study. Over the course of 1 year, bacteriologic cultures were performed on 434 of the 799 blood packs issued to children by the blood bank of Coast Provincial General Hospital, Mombasa. Clinical outcome was not assessed.

Results: Forty-four bacterial contaminants were isolated from 38 blood packs-an overall contamination frequency of 8.8% (95% confidence interval, 6.1%-11.4%). Sixty-four percent of the bacteria isolated were Gram-negative. Many of the isolates are usually found in the environment and the most likely source of contamination was considered to be the hospital blood bank.

Conclusion: Bacterial contamination of whole blood may be a significant but unrecognized hazard of blood transfusion for children in sub-Saharan Africa. Further work is needed to clarify the extent of the problem and its clinical consequences. Increased awareness and adherence to basic principles of asepsis in the hospital blood bank may be important immediate interventions.

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Figures

Fig. 1
Fig. 1
The number of blood packs issued to children, the number of blood packs cultured, and the number of blood packs from which bacteria were isolated (CPGH, February 2006 to January 2007).
See this image and copyright information in PMC

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