Review
doi: 10.1007/978-1-60761-103-5_11.
Cytological analysis of meiosis in Caenorhabditis elegans
Affiliations
- PMID: 19685325
- PMCID: PMC3644504
- DOI: 10.1007/978-1-60761-103-5_11
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Review
Cytological analysis of meiosis in Caenorhabditis elegans
Methods Mol Biol.
2009.
Abstract
The nematode Caenorhabditis elegans has emerged as an informative experimental system for analysis of meiosis, in large part because of the advantageous physical organization of meiotic nuclei as a gradient of stages within the germline. Here we provide tools for detailed observational studies of cells within the worm gonad, including techniques for light and electron microscopy.
Figures
Meiotic progression in C. elegans. (A) Diagram of a C. elegans hermaphrodite. The bi-lobed gonad contains a temporal progression of meiotic nuclei. (B) Whole gonad dissected out of a wild-type worm and stained with DAPI. The stages of meiotic prophase can be clearly identified by the chromatin morphology.
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