Cortisol secretion after adrenocorticotrophin (ACTH) and dexamethasone tests in healthy female and male dogs

Abstract

Background: For the conclusive diagnosis of Cushing's Syndrome, a stimulating ACTH test or a low suppressive Dexamethasone test is used. Reports in other species than the dog indicate that plasma cortisol concentration after ACTH administration is affected by gender. We investigated the effect of gender on the cortisol response to ACTH and Dexamethasone tests in dogs.

Methods: Seven healthy adult Cocker Spaniels (4 females and 3 males) were assigned to a two by two factorial design: 4 dogs (2 females and 2 males) received IV Dexamethasone 0.01 mg/kg, while the other 3 dogs received an IV saline solution (control group). Two weeks later the treatments were reversed. After one month, ACTH was given IV (250 microg/animal) to 4 dogs (2 female and 2 males) while the rest was treated with saline solution (control group). Cortisol concentrations were determined by a direct solid-phase radioimmunoassay and cholesterol and triglycerides by commercial kits.

Results and discussion: No effect of treatment was observed in metabolite concentrations, but females presented higher cholesterol concentrations. ACTH-treated dogs showed an increase in cortisol levels in the first hour after sampling until 3 hours post injection. Cortisol concentrations in Dexamethasone-treated dogs decreased one hour post injection and remained low for 3 hours, thereafter cortisol concentrations increased. The increase in cortisol levels from one to two hours post ACTH injection was significantly higher in females than males. In Dexamethasone-treated males cortisol levels decreased one hour post injection up to 3 hours; in females the decrease was more pronounced and prolonged, up to 5 hours post injection.

Conclusion: We have demonstrated that cortisol response to ACTH and Dexamethasone treatment in dogs differs according to sex.

Figure 1

Cortisol concentrations after ACTH, Dexamethasone and Saline solution treatments in healthy Cocker Spaniel dogs.

Figure 2

Cholesterol (A) and triglycerides (B) concentrations in healthy female and male Cocker Spaniel dogs. Open dots: females. Solid line: males.

Figure 3

Cortisol concentrations in female and male healthy Cocker Spaniel dogs after Dexamethasone (A), ACTH (B) and Saline solution (C) treatments. Observe that the scale in B panel is different from A and C panels. Open dots: females. Solid line: males.