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. 2009 Nov;53(11):4733-9.
doi: 10.1128/AAC.00533-09. Epub 2009 Aug 17.

Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in western Pennsylvania

Hanna E Sidjabat  1 , David L Paterson, Jennifer M Adams-Haduch, Lindsay Ewan, Anthony W Pasculle, Carlene A Muto, Guo-Bao Tian, Yohei Doi
Affiliations

Molecular epidemiology of CTX-M-producing Escherichia coli isolates at a tertiary medical center in western Pennsylvania

Hanna E Sidjabat et al. Antimicrob Agents Chemother. 2009 Nov.

Abstract

A combination of phenotypic and genotypic methods was used to investigate 70 unique Escherichia coli clinical isolates identified as producing extended-spectrum beta-lactamases (ESBLs) at a medical center in Pittsburgh, PA, between 2007 and 2008. Fifty-seven isolates (81%) produced CTX-M-type ESBLs, among which CTX-M-15 was predominant (n = 46). Isolates producing CTX-M-2, -9, -14, and -65 were also identified. One CTX-M-producing isolate coproduced CMY-2 cephalosporinase. Ten isolates (14%) produced SHV-type ESBLs, either SHV-5 or SHV-7. Two isolates produced only CMY-2 or -32. Pulsed-field gel electrophoresis revealed the presence of two major clusters of CTX-M-15-producing E. coli isolates, one in phylotype B2 (n = 15) and the other in phylotype A (n = 19). Of four phylotype B2 isolates that were able to transfer the bla(CTX-M-15)-carrying plasmids, three showed fingerprints related (>60%) to those of plasmids from phylotype A isolates. In phylotype B2, all CTX-M-15-producing isolates, as well as three isolates producing CTX-M-14, two producing SHV-5, and one producing SHV-7, belonged to the international epidemic clone defined by serotype O25:H4 and sequence type 131. The plasmids from eight of nine CTX-M-15-producing E. coli isolates of phylotype A that were examined were highly related to each other and were also found in two isolates belonging to phylotype D, suggesting horizontal transfer of this bla(CTX-M-15)-carrying plasmid between phylotypes. Our findings underscore the need to further investigate the epidemiology and virulence of CTX-M-producing E. coli in the United States.

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Figures

FIG. 1.
FIG. 1.
Dendrogram based on PFGE profiles of 70 E. coli isolates. Ph, phylotype; A, pulsotype A; B, pulsotype B.
FIG. 2.
FIG. 2.
Dendrogram of plasmids carrying blaCTX-M. Ph, phylotypes.
FIG. 3.
FIG. 3.
Profiles of HpaI-digested plasmids from CTX-M-producing E. coli isolates and Southern hybridization. (a) Plasmid profiles of transformants; (b) Southern hybridization of plasmids using a digoxigenin-labeled blaCTX-M-15 probe. M, λ/HindIII and 100-bp ladder (MBI Fermentas); Ph, phylotypes.
See this image and copyright information in PMC

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