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Comment
. 2009 Aug 19;28(16):2307-8.
doi: 10.1038/emboj.2009.221.

Measuring ubiquitin chain linkage: Rap80 uses a molecular ruler mechanism for ubiquitin linkage specificity

Kylie J Walters  1 , Xiang Chen
Affiliations
Comment

Measuring ubiquitin chain linkage: Rap80 uses a molecular ruler mechanism for ubiquitin linkage specificity

Kylie J Walters et al. EMBO J. .
No abstract available

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
Rap80 uses a molecular ruler mechanism to respond to K63-linked ubiquitin chains. (A) K63-linked ubiquitin chains at DNA damage sites attract Rap80, which brings a BRCA1 complex. Rap80's inter-UIM region acts as a molecular ruler by restricting its UIMs to a configuration optimized for binding to K63-linked ubiquitin chains. (B) Structure, solved by Sato et al., of Rap80-UIM1-UIM2:K63-linked diubiquitin is shown highlighting K48 (in grey) and the required changes for tandem binding with the M1 linkage (blue). E64 (shown in yellow) must rotate in M1-linked chains (data not shown) to avoid steric clashes with the linker region.
See this image and copyright information in PMC

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References

    1. Rahighi S, Ikeda F, Kawasaki M, Akutsu M, Suzuki N, Kato R, Kensche T, Uejima T, Bloor S, Komander D, Randow F, Wakatsuki S, Dikic I (2009) Specific recognition of linear ubiquitin chains by NEMO is important for NF-kappaB activation. Cell 136: 1098–1109 - PubMed
    1. Sato Y, Yoshikawa A, Mimura H, Yamashita M, Yamagata A, Fukai S (2009) Structural basis for specific recognition of Lys 63-linked polyubiquitin chains by tandem UIMs of RAP80. EMBO J 28: 2461–2468 - PMC - PubMed
    1. Sims JJ, Cohen RE (2009) Linkage-specific avidity defines the lysine 63-linked polyubiquitin-binding preference of rap80. Mol Cell 33: 775–783 - PMC - PubMed
    1. Sobhian B, Shao G, Lilli DR, Culhane AC, Moreau LA, Xia B, Livingston DM, Greenberg RA (2007) RAP80 targets BRCA1 to specific ubiquitin structures at DNA damage sites. Science 316: 1198–1202 - PMC - PubMed
    1. Ulrich HD (2009) Regulating post-translational modifications of the eukaryotic replication clamp PCNA. DNA Repair (Amst) 8: 461–469 - PubMed

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