The successful pregnancy and birth of a healthy baby after human blastocyst vitrification using Cryo-E, first case in Siriraj Hospital

Abstract

This case report illustrates a successful pregnancy and birth after vitrification of a human blastocyst using modified cryostorage- Cryo E. A 31-year-old woman underwent short protocol for ovarian hyperstimulation in an IVF treatment program. After administration of intramuscular hCG, all oocytes were recovered transvaginally with ultrasound guidance. Twelve mature oocytes were obtained and eight were fertilized Three out of eight embryos became blastocyst, and two blastocysts were transferred on day 5, but no implantation occurred The other was cryopreserved by vitrification technique using a cryo E. Three months after freezing, the cryopreserved blastocyst was thawed and survived. It was transferred to the patient's uterus. Blastocyst implantation resulted in a healthy single pregnancy. After continuing pregnancy until term, she delivered by cesarean section due to unfavorable cervical conditions. A male baby with Apgar score 9/10, weighing 3500 g was born. Her child showed normal growth.