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. 1990 Mar 11;18(5):1129-32.
doi: 10.1093/nar/18.5.1129.

Large restriction fragments containing poly-TG are highly polymorphic in a variety of vertebrates

Y Kashi  1 , Y Tikochinsky, E Genislav, F Iraqi, A Nave, J S Beckmann, Y Gruenbaum, M Soller
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Free PMC article

Large restriction fragments containing poly-TG are highly polymorphic in a variety of vertebrates

Y Kashi et al. Nucleic Acids Res. .
Free PMC article

Abstract

Southern blots of genomic DNA from a variety of species digested by restriction endonucleases having a four-bp specificity, were probed with a bovine genomic clone consisting of seven tandem poly-TG stretches separated by a 29bp linker sequence. Highly variable DNA 'fingerprint' patterns were obtained in chicken, sheep, and horse, moderately variable DNA 'fingerprints' in mouse and man, and a monomorphic pattern in Drosophila. In chicken, horse and man a (TG)10 synthetic oligonucleotide probe gave results identical to those given by the bovine probe. Furthermore, in chicken the DNA fingerprint variation showed typical Mendelian inheritance and differed from the fingerprints obtained with Jeffreys 33.6 and M13 minisatellite probes. Thus, for a variety of vertebrate species, poly-TG-containing probes can uncover useful genetic variation.

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References

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