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. 1990 Apr 9;50(1):45-51.
doi: 10.1016/0304-3835(90)90177-y.

Novel screening method for agents that overcome classical multidrug resistance in a human cell line

A Yoshimura  1 , N Shudo, S I Ikeda, M Ichikawa, T Sumizawa, S I Akiyama
Affiliations

Novel screening method for agents that overcome classical multidrug resistance in a human cell line

A Yoshimura et al. Cancer Lett. .

Abstract

P-glycoprotein (P-gp) is involved in the transport of a wide variety of organic compounds including a fluorescent dye, rhodamine 6G (RG), as well as anti-cancer drugs. Agents that overcome classical multidrug-resistance (MDR) increased the accumulation of RG in a MDR cell line, KB-C1. The effect of agents on RG-accumulation in KB-C1 cells was highly correlated with their effect on the reversion of vincristine-resistance in KB-C1 cells. RG was detected on a fluorescence microplate reader with a rhodamine channel. This assay may become a useful method for the screening of agents that overcome classical MBR, since it is quick and simple.

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