Sympathetic noradrenergic neurons containing dynorphin but not neuropeptide Y innervate small cutaneous blood vessels of guinea-pigs

Abstract

We have used double-labelling immunofluorescence techniques and retrograde axonal transport of Fast Blue to characterize three populations of sympathetic noradrenergic neurons innervating blood vessels in the hairless skin of the ears and paws of guinea-pigs. Each population of neurons innervated a specific level of the vascular bed, and had a distinctive content of neuropeptides. Sympathetic noradrenergic neurons innervating large distributing arteries contained immunoreactivity to neuropeptide Y. Neurons innervating smaller cutaneous arteries contained immunoreactivity to prodynorphin-derived peptides in addition to neuropeptide Y. Finally, sympathetic neurons innervating the smallest arterioles, and arterio-venous anastomoses, contained immunoreactivity to prodynorphin-derived peptides, but had no detectable neuropeptide Y. Although the major form of dynorphin immunoreactivity in perivascular sympathetic axons was dynorphin A(1-8), immunoreactivity to both dynorphin A(1-8) and dynorphin A(1-17) occurred in the cell bodies of these neurons, suggesting that dynorphin A is processed during axonal transport to the terminals. The perivascular sympathetic neurons containing prodynorphin-derived peptides but not neuropeptide Y are most likely to be involved in the regulation of thermoregulatory cutaneous vascular circuits.