[Results of the VNTR-analysis in a locus (5'-NAAA-3')n of strains Yersinia pestis from the active natural foci of plague of Siberia]

Abstract

The method of the VNTR-analysis in a locus (5'-NAAA-3')n was studied using samples of the DNA of 73 strains Y. pestis ssp. altaica, isolated in Gorno-Altaisk and 65 strains Y. pestis ssp. Pestis, allocated in Tuva natural foci of plague. It was demonstrated that strains from the Gorno-Altaisk population of plague microbes possessed genomic polymorphism in the locus given a marker that is reflected in variability of the size of an amplicon from 250 up to 275 bp, correlating with places of isolation of strains in separate epizootic loci. Strains Y. pestis ssp. pestis from the Tuva population, on the contrary, are characterized by conservatism in the locus 5'-NAAA-3' (the size of an amplicon was 261 bp). Providing selective direct sequencing of amplicons, exact values of their sizes and number of repeats of a tetranucleotide 5'-NAAA-3' were determined.