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. 1990 Apr;32(1):63-70.
doi: 10.1016/0165-0270(90)90072-n.

A chemically-defined medium for organotypic slice cultures

C M Annis  1 , J Edmond, R T Robertson
Affiliations

A chemically-defined medium for organotypic slice cultures

C M Annis et al. J Neurosci Methods. 1990 Apr.

Abstract

Organotypic slice cultures provide an excellent system for the analysis of study of the molecular mechanisms of this development necessitates the use of a chemically defined culture medium. We report here the development of a medium, EOL1 defined medium, designed specifically for this purpose. Cultures of both cerebral cortex and basal forebrain demonstrate that this defined medium allows a high degree of cytoarchitectural maintenance while promoting neural metabolism and process outgrowth.

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