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. 2009 Dec;58(12):2749-56.
doi: 10.2337/db09-0369. Epub 2009 Aug 31.

Impact of 9 days of bed rest on hepatic and peripheral insulin action, insulin secretion, and whole-body lipolysis in healthy young male offspring of patients with type 2 diabetes

Amra C Alibegovic  1 , Lise Højbjerre, Mette P Sonne, Gerrit van Hall, Bente Stallknecht, Flemming Dela, Allan Vaag
Affiliations

Impact of 9 days of bed rest on hepatic and peripheral insulin action, insulin secretion, and whole-body lipolysis in healthy young male offspring of patients with type 2 diabetes

Amra C Alibegovic et al. Diabetes. 2009 Dec.

Abstract

Objective: The aim of this study was to investigate the impact of 9 days of bed rest on insulin secretion, insulin action, and whole-body glucose and fat metabolism in first-degree relative (FDR) and matched control (CON) subjects.

Research design and methods: A total of 13 FDR and 20 CON subjects participated in the study. All were studied before and after 9 days of bed rest using the clamp technique combined with indirect calorimetry preceded by an intravenous glucose tolerance test. Glucose and glycerol turnover rates were studied using stable isotope kinetics.

Results: Bed rest caused a significant decrease in whole-body insulin sensitivity in both groups. Hepatic insulin resistance was elevated in FDR subjects prior to bed rest and was significantly augmented by bed rest in FDR (P < 0.01) but not in CON (P = NS) subjects. The rate of whole-body lipolysis decreased during bed rest in both FDR and CON subjects, with no significant differences between the groups. Insulin resistance induced by bed rest was fully accounted for by the impairment of nonoxidative glucose metabolism in both groups (overall P < 0.001).

Conclusions: Whole-body insulin action in both insulin-resistant FDR and healthy CON subjects deteriorates with 9 days of bed rest, converging toward similar degrees of whole-body insulin resistance. FDR subjects exhibit hepatic insulin resistance (HIR), which, in contrast to CON subjects, deteriorates in response to physical inactivity. FDR subjects exhibit reduced insulin secretion when seen in relation to their degree of HIR but not peripheral insulin resistance.

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Figures

FIG. 1.
FIG. 1.
Study outline. The experimental protocol presented as a figure showing activities undertaken during the study.
FIG. 2.
FIG. 2.
The average glucose infusion rate (M value) in response to 9 days of bed rest in CON and FDR subjects, respectively. The solid line represents the average M value change in CON and FDR subjects in response to bed rest.
FIG. 3.
FIG. 3.
Fasting plasma insulin during 9 days of bed rest. *P < 0.05. Data are presented as means ± SE in FDR (▾) and CON (▿) subjects. Fasting plasma C-peptide during 9 days of bed rest. *P < 0.05. Data are presented as means ± SE in FDR (●) and CON (○) subjects.
FIG. 4.
FIG. 4.
HIR index. *P < 0.01; **P < 0.001; #significant increase of HIR in response to bed rest. Data are presented as means ± SEM in FDR (■) and CON (□) subjects; abr, after bed rest; bbr, before bed rest.
See this image and copyright information in PMC

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