Comparative Study
doi: 10.1590/s0074-02762009000400007.
Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods
Affiliations
- PMID: 19722079
- DOI: 10.1590/s0074-02762009000400007
Item in Clipboard
Comparative Study
Comparative study of two extraction methods for enteric virus recovery from sewage sludge by molecular methods
Mem Inst Oswaldo Cruz.
2009 Jul.
Free article
Abstract
The aim of this study was to compare two nucleic acid extraction methods for the recovery of enteric viruses from activated sludge. Test samples were inoculated with human adenovirus (AdV), hepatitis A virus (HAV), poliovirus (PV) and rotavirus (RV) and were then processed by an adsorption-elution-precipitation method. Two extraction methods were used: an organic solvent-based method and a silica method. The organic-based method was able to recoup 20% of the AdV, 90% of the RV and 100% of both the PV and HAV from seeded samples. The silica method was able to recoup 1.8% of the AdV and 90% of the RV. These results indicate that the organic-based method is more suitable for detecting viruses in sewage sludge.
Similar articles
-
Detection of enteric viruses in sewage sludge and treated wastewater effluent.Water Sci Technol. 2010;61(2):537-44. doi: 10.2166/wst.2010.845. Water Sci Technol. 2010. PMID: 20107281
-
Detection of enteroviruses, hepatitis A virus and rotaviruses in sewage by means of an immunomagnetic capture reverse transcription-PCR assay.Microbiol Res. 2002;157(3):169-75. doi: 10.1078/0944-5013-00152. Microbiol Res. 2002. PMID: 12398285
-
Concentration of enteric viruses from tap water using an anion exchange resin-based method.J Virol Methods. 2014 Sep;206:95-8. doi: 10.1016/j.jviromet.2014.05.025. Epub 2014 Jun 6. J Virol Methods. 2014. PMID: 24911889
-
The efficiency of concentration methods used to detect enteric viruses in anaerobically digested sludge.Mem Inst Oswaldo Cruz. 2013 Feb;108(1):77-83. doi: 10.1590/s0074-02762013000100013. Mem Inst Oswaldo Cruz. 2013. PMID: 23440119 Free PMC article.
-
Detection of poliovirus, hepatitis A virus and rotavirus from sewage and water samples.Southeast Asian J Trop Med Public Health. 2000 Mar;31(1):41-6. Southeast Asian J Trop Med Public Health. 2000. PMID: 11023063
Cited by
-
Performance evaluation of virus concentration methods for implementing SARS-CoV-2 wastewater based epidemiology emphasizing quick data turnaround.Sci Total Environ. 2021 Dec 20;801:149656. doi: 10.1016/j.scitotenv.2021.149656. Epub 2021 Aug 14. Sci Total Environ. 2021. PMID: 34418628 Free PMC article.
-
The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019.Sci Total Environ. 2021 Jul 15;778:146198. doi: 10.1016/j.scitotenv.2021.146198. Epub 2021 Mar 8. Sci Total Environ. 2021. PMID: 33714813 Free PMC article.
-
Assessing sensitivity and reproducibility of RT-ddPCR and RT-qPCR for the quantification of SARS-CoV-2 in wastewater.J Virol Methods. 2021 Nov;297:114230. doi: 10.1016/j.jviromet.2021.114230. Epub 2021 Jul 9. J Virol Methods. 2021. PMID: 34252511 Free PMC article.
-
A novel easy-to-desorb eluant contributes to address environmental contamination of African swine fever virus.AMB Express. 2024 May 10;14(1):55. doi: 10.1186/s13568-024-01697-1. AMB Express. 2024. PMID: 38730054 Free PMC article.
-
Insights from a Systematic Search for Information on Designs, Costs, and Effectiveness of Poliovirus Environmental Surveillance Systems.Food Environ Virol. 2017 Dec;9(4):361-382. doi: 10.1007/s12560-017-9314-4. Epub 2017 Jul 7. Food Environ Virol. 2017. PMID: 28687986 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
