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. 2009 May 8;112(22):8251-8258.
doi: 10.1021/jp801293a.

Spontaneous Formation of Functionalized Dendrimer-Stabilized Gold Nanoparticles

Xiangyang Shi  1 , Kai Sun, James R Baker
Affiliations

Spontaneous Formation of Functionalized Dendrimer-Stabilized Gold Nanoparticles

Xiangyang Shi et al. J Phys Chem C Nanomater Interfaces. .

Abstract

Functionalized, dendrimer-stabilized gold nanoparticles (Au DSNPs) are of scientific and technological interest for biological applications. In this work, we show that acetamide-functionalized Au DSNPs can be formed by acetylation of amine-terminated poly(amidoamine) (PAMAM) dendrimers of generation 5 (G5.NH(2)) complexed with Au(III) ions (AuCl(4) (-)). In addition, hydroxyl-functionalized Au DSNPs can be formed by simply mixing the glycidol hydroxyl-terminated G5 dendrimers (G5.NGlyOH) with HAuCl(4). In both cases, no additional reducing agents were needed and the reactions were completed at room temperature. We also show that Alexa Fluor 594 dye-functionalized Au DSNPs can be formed by acetylation of Alexa Fluor 594-conjugated, amine-terminated G5 dendrimers complexed with HAuCl(4). All of these functionalized Au DSNPs are water-soluble and stable. Fluorescence spectroscopy studies reveal that the Alexa Fluor 594-functionalized Au DSNPs retain similar fluorescence intensity to the Alexa Fluor 594-functionalized dendrimers that lack Au nanoparticles. These preparations of Au DSNPs provide a straightforward approach to synthesizing functionalized metal nanoparticles for biomedical applications.

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Figures

Figure 1
Figure 1
UV–vis spectra of the formed {(Au3+)7-G5.NH2} complexes (curve 1); {(Au0)7-G5.NHAc} DSNPs (curve 2); {(Au3+)7-G5.NH2} complexes after reaction with glycidol for 24 h (curve 3); {(Au0)7-G5.NGlyOH} DSNPs (curve 4); and {(Au0)7-G5.NGlyOH} DSNPs formed by addition of additional glycidol (curve 5). The inset shows the photographs of the corresponding solutions of {(Au3+)7-G5.NH2} complexes (1); {(Au0)7-G5.NHAc} DSNPs (2); and {(Au0)7-G5.NGlyOH} DSNPs (4).
Figure 2
Figure 2
1H NMR spectra of {(Au3+)7-G5.NH2} complexes (a) and {(Au0)7-G5.NHAc} DSNPs (b). A schematic representation of the dendrimer structure used for NMR assignment is shown at the bottom.
Figure 3
Figure 3
1H NMR spectra of G5.NGlyOH dendrimers (a) and {(Au0)7-G5.NGlyOH} DSNPs (b). A schematic representation of the dendrimer structure used for NMR assignment is shown at the bottom.
Figure 4
Figure 4
TEM image (a), size distribution histogram (b), selected area electron diffraction pattern (c), and EDS spectrum (d) of {(Au0)7-G5.NHAc} DSNPs.
Figure 5
Figure 5
TEM image (a), size distribution histogram (b), and high-resolution TEM image (c) of {(Au0)7-G5.NGlyOH} DSNPs.
Figure 6
Figure 6
TEM image (a) and size distribution histogram (b) of {(Au0)10-G5.NHAc-AF594} DSNPs.
Figure 7
Figure 7
UV–vis (a) and fluorescence (b) spectra of G5.NHAc-AF594 dendrimers and {(Au0)10-G5.NHAc-AF594} DSNPs.
SCHEME 1
SCHEME 1. Schematic Representation of the Approaches to Synthesizing {(Au0)7-G5.NHAc} (a), {(Au0)7-G5.NGlyOH} (b), and {(Au0)10-G5.NHAc-AF594} DSNPs (c)
See this image and copyright information in PMC

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