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. 1977;4(5):1539-52.
doi: 10.1093/nar/4.5.1539.

Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide

J Casey, N Davidson
Free PMC article

Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide

J Casey et al. Nucleic Acids Res. 1977.
Free PMC article

Abstract

The thermal stabilities of RNA:DNA hybrids are substantially greater than those of DNA:DNA duplexes in aqueous electrolyte solutions containing high concentrations of formamide. Association rates to form DNA:DNA duplexes and DNA:RNA hybrids have been measured in these solvents. There is a temperature range in which DNA:DNA rates are negligible and RNA:DNA rates close to optimal.

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References

    1. Anal Biochem. 1976 Jan;70(1):75-85 - PubMed
    1. J Mol Biol. 1962 Jul;5:109-18 - PubMed
    1. J Mol Biol. 1975 Mar 5;92(3):433-48 - PubMed
    1. Biochemistry. 1974 Dec 31;13(27):5467-73 - PubMed
    1. Biochemistry. 1972 Mar 28;11(7):1319-26 - PubMed

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