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. 2009 Nov;75(21):6937-40.
doi: 10.1128/AEM.01742-09. Epub 2009 Sep 11.

Composition and activity of an autotrophic Fe(II)-oxidizing, nitrate-reducing enrichment culture

Marco Blöthe  1 , Eric E Roden
Affiliations

Composition and activity of an autotrophic Fe(II)-oxidizing, nitrate-reducing enrichment culture

Marco Blöthe et al. Appl Environ Microbiol. 2009 Nov.

Abstract

16S rRNA gene libraries from the lithoautotrophic Fe(II)-oxidizing, nitrate-reducing enrichment culture described by Straub et al. (K. L. Straub, M. Benz, B. Schink, and F. Widdel, Appl. Environ. Microbiol. 62:1458-1460, 1996) were dominated by a phylotype related (95% 16S rRNA gene homology) to the autotrophic Fe(II) oxidizer Sideroxydans lithotrophicus. The libraries also contained phylotypes related to known heterotrophic nitrate reducers Comamonas badia, Parvibaculum lavamentivorans, and Rhodanobacter thiooxidans. The three heterotrophs were isolated and found to be capable of only partial (12 to 24%) Fe(II) oxidation, suggesting that the Sideroxydans species has primary responsibility for Fe(II) oxidation in the enrichment culture.

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Figures

FIG. 1.
FIG. 1.
(A) Cell growth and Fe(II) oxidation by the enrichment culture with nitrate as the electron acceptor. (B) Fe(II) oxidation with nitrite as the electron acceptor. Data are presented as means ± standard deviations (SD) for triplicate cultures. Filled and open symbols represent results from inoculated and uninoculated cultures, respectively. • and ○, Fe(II); ▴ and ▵, nitrate; ▪ and □, nitrite; ×, cell numbers.
FIG. 2.
FIG. 2.
Response of a freshly grown (ca. 1-week-old) enrichment culture to the addition of 8 mM Fe(II) (refeed) (A) or a combination of 0.5% yeast extract, 0.5% tryptic soy broth, and 0.5 mM acetate (add organics) (B). Data are presented as means ± SD for triplicate cultures. Symbols are as in Fig. 1.
FIG. 3.
FIG. 3.
Fe redox cycling by the enrichment culture and G. sulfurreducens driven by alternating addition of acetate and nitrate. Cocultures were initiated with either Fe(II) oxidation (A to C) or Fe(III) reduction (D to F). Data are presented as means ± SD for triplicate cultures. Symbols are as in Fig. 1; acetate, ▾.
See this image and copyright information in PMC

References

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