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. 1977 Jul;74(7):2780-4.
doi: 10.1073/pnas.74.7.2780.

Crosslinked histone octamer as a model of the nucleosome core

A Stein, M Bina-Stein, R T Simpson

Crosslinked histone octamer as a model of the nucleosome core

A Stein et al. Proc Natl Acad Sci U S A. 1977 Jul.

Abstract

When histones in chromatin core particles were crosslinked with dimethylsuberimidate, the resulting particles had properties closely similar to those of native core particles. A crosslinked octameric histone complex was isolated from these particles under nondenaturing conditions. Upon reaction with DNA, this octameric protein folded the DNA into a structure closely resembling that of native core particles as verified by various techniques; protein denaturants were necessary for reassociation. The histone octamer is useful as a model of the nucleosome protein core and for studying histone-DNA interactions that occur in nucleosomes.

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References

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    1. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4280-4 - PubMed
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