Effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) treatment in vivo on thymocyte functions in mice after activation in vitro

Abstract

Thymocytes from 15-day old C57BL/6 mice, pretreated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) 4 days before sacrifice, showed an earlier response and a higher maximal cell proliferation than thymocytes from control mice upon stimulation by concanavalin A in vitro. This is partly in contrast to the conclusions from earlier published studies. IL-2 content--as measured by growth of CTLL cells--was equally high in TCDD and in control cultures at day 1. At day 2, TCDD cultures had decreased dramatically in IL-2 content, possibly due to a high rate of consumption. At this point in time, the controls still contained a high concentration of IL-2, although less than at day 1. In contrast to the increased sensitivity to mitogen stimulation, thymocytes from TCDD-treated mice induced B-cells less avidly with respect to antibody production, and could also inhibit the T-cell help of thymocytes from untreated animals, a phenomenon which could be reversed if TCDD-treated thymocytes were irradiated before culture.