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Case Reports
. 2009 Nov;47(11):3617-23.
doi: 10.1128/JCM.00539-09. Epub 2009 Sep 16.

Molecular and phenotypic characteristics of methicillin-resistant and vancomycin-intermediate staphylococcus aureus isolates from patients with septic arthritis

Wei-Yao Wang  1 , Shih-Yi Lee, Tzong-Shi Chiueh, Jang-Jih Lu
Affiliations
Case Reports

Molecular and phenotypic characteristics of methicillin-resistant and vancomycin-intermediate staphylococcus aureus isolates from patients with septic arthritis

Wei-Yao Wang et al. J Clin Microbiol. 2009 Nov.

Abstract

Staphylococcus aureus is one of the most common pathogens in community- and hospital-associated infections and frequently causes severe and intractable infections in osteoarticular tissues. S. aureus isolates that are resistant to methicillin (meticillin) (MRSA isolates) and intermediately resistant to vancomycin (VISA isolates) have emerged. In this report, we described two patients, one female and one male, diagnosed with septic arthritis due to S. aureus infections. A total of 13 MRSA isolates were obtained from these two patients. All but one isolate belonged to the VISA group. All seven isolates from the female patient were determined to be community associated, multilocus sequence type (MLST) 59, and staphylococcal cassette chromosome mecA (SCCmec) type IV; had direct repeat units (DRUs) of nine repeats; were spa type t437; and were susceptible to sulfa and quinolone antibiotics. The other six isolates, from the male patient, were determined to be hospital associated, MLST 239, and SCCmec type III; had DRUs of 14 repeats; were spa type t037; and were resistant to sulfa and quinolone antibiotics. All 13 MRSA isolates were in agr group I, were pvl negative, and showed no evidence of any association between vancomycin resistance and autolysis.

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Figures

FIG. 1.
FIG. 1.
PFGE of MRSA isolates from patients with septic arthritis. (A) CA-MRSA isolates from the 67-year-old female patient. M, marker; lane 1, strain no. 109; lane 2, strain no. 1962; lane 3, strain no. 2357; lane 4, strain no. 2358; lane 5, strain no. 2459; lane 6, strain no. 2482; lane 7, strain no. 2516. (B) HA-MRSA isolates from the 19-year-old male patient. M, marker; lane 1, strain no. 188; lane 2, strain no. 204; lane 3, strain no. 218; lane 4, strain no. 257.
FIG. 2.
FIG. 2.
Population analysis of the CA-MRSA isolates from the 67-year-old female patient with septic arthritis (strain no. 109, VSSA; strain no. 1962, h-VISA; strains 2357, 2358, 2459, 2482, and 2516, VISA; Mu50, VISA reference strain; Mu3, h-VISA reference strain; ATCC 25923, VSSA reference strain).
FIG. 3.
FIG. 3.
Autolysis study of the CA-MRSA isolates from the 67-year-old female patient with septic arthritis (strain no. 109, VSSA; strain no. 1962, h-VISA; strain no. 2357, 2358, 2459, 2482, and 2516, VISA; Mu50, VISA reference strain; ATCC 25923, VSSA reference strain).
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References

    1. Al-Nammari, S. S., P. Bobak, and R. Venkatesh. 2007. Methicillin resistant Staphylococcus aureus versus methicillin sensitive Staphylococcus aureus adult haematogenous septic arthritis. Arch. Orthop. Trauma Surg. 127:537-542. - PubMed
    1. Bocchini, C. E., K. G. Hulten, E. O. Mason, Jr., B. E. Gonzalez, W. A. Hammerman, and S. L. Kaplan. 2006. Panton-Valentine leukocidin genes are associated with enhanced inflammatory response and local disease in acute hematogenous Staphylococcus aureus osteomyelitis in children. Pediatrics 117:433-440. - PubMed
    1. Boyle-Vavra, S., and R. S. Daum. 2007. Community-acquired methicillin-resistant Staphylococcus aureus: the role of Panton-Valentine leukocidin. Lab. Investig. 87:3-9. - PubMed
    1. Chen, C. J., C. H. Chiu, T. Y. Lin, Z. L. Lee, W. E. Yang, and Y. C. Huang. 2007. Experience with linezolid therapy in children with osteoarticular infections. Pediatr. Infect. Dis. J. 26:985-988. - PubMed
    1. CLSI. 2009. Performance standards for antimicrobial disk susceptibility tests. Approved standard (M02-A10). CLSI, Wayne, PA.

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