Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Oct;172(10):5783-8.
doi: 10.1128/jb.172.10.5783-5788.1990.

Cloning and expression of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity

R K Jayaswal  1 , Y I Lee, B J Wilkinson
Affiliations

Cloning and expression of a Staphylococcus aureus gene encoding a peptidoglycan hydrolase activity

R K Jayaswal et al. J Bacteriol. 1990 Oct.

Abstract

A gene of Staphylococcus aureus PS47 encoding lytic activity was cloned and expressed in Escherichia coli. Deletion analysis of a recombinant plasmid carrying a 7.4-kilobase-pair fragment (kbp) of S. aureus DNA suggested that the gene was located within a 2.5-kbp EcoRI-XbaI fragment. Analysis of extracts of E. coli harboring recombinant plasmids on denaturing polyacrylamide gels containing purified cell walls of S. aureus showed a clearing zone by a polypeptide of apparent Mr 23,000. The release of dinitrophenylalanine but not reducing groups from purified cell walls by a cell extract of recombinant E. coli suggested that we had cloned an N-acetylmuramyl-L-alanine amidase.

PubMed Disclaimer

References

    1. J Bacteriol. 1967 Mar;93(3):1089-95 - PubMed
    1. J Bacteriol. 1964 Sep;88:676-81 - PubMed
    1. Anal Biochem. 1968 Feb;22(2):260-8 - PubMed
    1. J Bacteriol. 1969 Feb;97(2):837-47 - PubMed
    1. J Bacteriol. 1970 Sep;103(3):761-9 - PubMed

Publication types

MeSH terms

LinkOut - more resources