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Comparative Study
. 2009 Sep;46(5):1074-83.
doi: 10.1603/033.046.0514.

Genetic structure of Aedes aegypti in Australia and Vietnam revealed by microsatellite and exon primed intron crossing markers suggests feasibility of local control options

N M Endersby  1 , A A Hoffmann, V L White, S Lowenstein, S Ritchie, P H Johnson, L P Rapley, P A Ryan, V S Nam, N T Yen, P Kittiyapong, A R Weeks
Affiliations
Comparative Study

Genetic structure of Aedes aegypti in Australia and Vietnam revealed by microsatellite and exon primed intron crossing markers suggests feasibility of local control options

N M Endersby et al. J Med Entomol. 2009 Sep.

Abstract

The distribution of Aedes aegypti (L.) in Australia is currently restricted to northern Queensland, but it has been more extensive in the past. In this study, we evaluate the genetic structure of Ae. aegypti populations in Australia and Vietnam and consider genetic differentiation between mosquitoes from these areas and those from a population in Thailand. Six microsatellites and two exon primed intron crossing markers were used to assess isolation by distance across all populations and also within the Australian sample. Investigations of founder effects, amount of molecular variation between and within regions and comparison of F(ST) values among Australian and Vietnamese populations were made to assess the scale of movement ofAe. aegypti. Genetic control methods are under development for mosquito vector populations including the dengue vector Ae. aegypti. The success of these control methods will depend on the population structure of the target species including population size and rates of movement among populations. Releases of modified mosquitoes could target local populations that show a high degree of isolation from surrounding populations, potentially allowing new variants to become established in one region with eventual dispersal to other regions.

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Figures

Fig. 1
Fig. 1
Map of areas where Ae. aegypti was sampled in Australia, Vietnam, and Thailand. Inset figures: (a) collection sites in Thailand and Vietnam and (b) collection sites in north Queensland.
Fig. 2
Fig. 2
Factorial correspondence analysis by population for Ae. aegypti from Australia, Thailand, and Vietnam. Each point represents a sample region weighted by number of individuals and the sum of alleles present. Closed squares, Vietnam population samples; closed diamonds, Australian population samples; open circle, Thailand sample. Percentage of variation explained by the first factor = 32.36%.
Fig. 3
Fig. 3
Regression of Slatkin's linearized FST (FST/1 − FST) against the natural logarithm of geographical distance (km) for (a) all pairs of populations from Australia, Vietnam, and Thailand (Mantel r = 0.662, P < 0.001; R2 = 0.422, F = 123.462, P < 0.001); and (b) population pairs from Australia only (Mantel r = 0.371, P = 0.026; R2 = 0.208, F = 16.806, P < 0.001).
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