[Effects of Dan-Chuan-Hong decoction on myocardial apoptosis of acute myocardial ischemia in rats]

Abstract

Objective: To observe the effects of Dan-Chuan-Hong decoction on myocardial apoptosis of acute myocardial ischemia in rats.

Methods: Rats received Guan-Xin-Er-Hao, Dan-Chuan-Hong or normal saline at 30 minutes before ischemia. The model of acute myocardial infarction (AMI) in rats was set up by ligating the descending branch of left coronary artery. 3 hours after AMI, the hearts were isolated to detect the apoptosis of myocardial ischemia by TUNEL; 24 hours after AMI, the hearts were isolated to determine the area of myocardial infarction by quantitative histological assay (triphenyltetrazolium chloride, TTC stain).

Results: Comparing with model group,the myocardial infarct size and apoptosis index of all the treatment groups decreased. Comparing with Guan-Xin-Er-Hao (30 g/kg) or Dan-Chuan-Hong (10 g/kg), the myocardial infarct size and apoptosis index of Dan-Chuan-Hong (20 g/kg) had no statistic difference. And there was statistic difference between Guan-Xin-Er-Hao (30 g/kg)and Dan-Chuan-Hong (10 g/kg).

Conclusion: Comparing with Guan-Xin-Er-Hao, the decoction of Dan-Chuan-Hong has similar effect, which could protect hearts from myocardial ischemia by inhibiting the myocardial apoptosis.