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. 2010 Mar;36(2):192-204.
doi: 10.1016/j.burns.2009.06.211. Epub 2009 Sep 24.

A microarray analysis of temporal gene expression profiles in thermally injured human skin

J A Greco 3rd  1 , A C Pollins, B E Boone, S E Levy, L B Nanney
Affiliations

A microarray analysis of temporal gene expression profiles in thermally injured human skin

J A Greco 3rd et al. Burns. 2010 Mar.

Abstract

Partial-thickness burns incite a multitude of responses which eventually culminate in cutaneous wound repair. We hypothesized that these events would evoke extensive alterations in gene expression thereby orchestrating the complexity of spatial and temporal events that characterize "normal" human wound healing. In the present study, gene expression from partial-thickness areas at defined temporal periods (1-3 days, 4-6 days, and 7-18 days) after injury were compared to normal non-wounded skin. Gene alterations proved extensive (2286 genes). Statistically significant alterations were noted among increased and decreased genes expressed in the three different temporal groupings. Our foundational data (based on samples from 45 individuals) provide a comprehensive molecular gene expression portrait of the cutaneous reparative responses that are initiated during the first 17 days after injury. Our efforts also represent an initial endeavor to move beyond the historically defined "morphological phases" of wound repair toward reporting molecular clues that define the temporal sequence of healing in human subjects. Further analysis of genes that are either affected or remain not affected following injury to normal skin is expected to identify potential targets for therapeutic augmentation or silencing.

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Figures

FIGURE 1
FIGURE 1
Number of significantly altered genes broken down by time group determined by Welch’s t-test with a Benjamini-Hochberg multiple corrections testing yielding p-values < 0.05 as significant. The large circle top-left represents the early time group (0-3 days), top-right represents the middle time group (4-7 days), and bottom-center represents the late time group (>7 days). Overlapping sections of the Venn diagram demonstrate individual genes whose expression levels are significantly altered in more than one time group.
FIGURE 2
FIGURE 2
A: Schematic representing the numerical breakdown of upregulated genes across all three time groups. Percentage of total number of upregulated genes (N=1136) also shown. B: Schematic representing the numerical breakdown of down-regulated genes across all three time groups. Percentage of total number of down-regulated genes (N=1003) also shown
See this image and copyright information in PMC

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