Collection of Macaca fascicularis cDNAs derived from bone marrow, kidney, liver, pancreas, spleen, and thymus

Abstract

Background: Consolidating transcriptome data of non-human primates is essential to annotate primate genome sequences, and will facilitate research using non-human primates in the genomic era. Macaca fascicularis is a macaque monkey that is commonly used for biomedical and ecological research.

Findings: We constructed cDNA libraries of Macaca fascicularis, derived from tissues obtained from bone marrow, liver, pancreas, spleen, and thymus of a young male, and kidney of a young female. In total, 5'-end sequences of 56,856 clones were determined. Including the previously established cDNA libraries from brain and testis, we have isolated 112,587 cDNAs of Macaca fascicularis, which correspond to 56% of the curated human reference genes.

Conclusion: These sequences were deposited in the public sequence database as well as in-house macaque genome database http://genebank.nibio.go.jp/qfbase/. These data will become valuable resources for identifying functional parts of the genome of macaque monkeys in future studies.

Figure 1

Number of BLAST hits (cutoff: 1e-60) against the human RefSeq genes. The grey bars represent the number of macaque ESTs matched to the human RefSeq genes. ESTs matched more than nine RefSeq genes were combined into a single bin. The red circles and lines represent the average nucleotide sequence identity between the macaque ESTs and RefSeq genes, ordered by the rank of BLAST hits. For example, the sequence identity in the second bin represents the sequence identity between the second best hits.