Genetic transformation of glutamine auxotrophy to prototrophy in the cyanobacterium Nostoc muscorum
- PMID: 1978772
- DOI: 10.1007/BF00276541
Genetic transformation of glutamine auxotrophy to prototrophy in the cyanobacterium Nostoc muscorum
Abstract
Glutamine auxotrophic (Gln-) and L-methionine D,L-sulfoximine (MSX) resistant (MSXr) mutants of N. muscorum were isolated and characterized for nitrogen nutrition, nitrogenase activity, glutamine synthetase (GS) activity and glutamine amide, alpha-keto-glutarate amido transferase (GOGAT) activity. The glutamine auxotroph was found to the GOGAT-containing GS-defective, incapable of growth with N2 or NH4+ but capable of growth with glutamine as nitrogen source, thus, suggesting GS to be the primary enzyme of both ammonia assimilation and glutamine formation in the cyanobacterium. The results of transformation and reversion studies suggests that glutamine auxotrophy is the result of a mutation in the gln A gene and that gln A gene can be transferred from one strain to another by transformation.
Similar articles
-
Regulation of glutamate dehydrogenase activity and ammonia production in a nitrogen fixing cyanobacterium.Acta Microbiol Hung. 1986;33(2):111-6. Acta Microbiol Hung. 1986. PMID: 2880448
-
Assimilation of 13NH4+ by Azospirillum brasilense grown under nitrogen limitation and excess.J Bacteriol. 1987 Sep;169(9):4211-4. doi: 10.1128/jb.169.9.4211-4214.1987. J Bacteriol. 1987. PMID: 2887545 Free PMC article.
-
Evidence for a role of glutamine synthetase in assimilation of amino acids as nitrogen source in the cyanobacterium Nostoc muscorum.Biochem Int. 1991 Dec;25(5):887-94. Biochem Int. 1991. PMID: 1687107
-
Ammonium assimilation in cyanobacteria.Photosynth Res. 2005;83(2):135-50. doi: 10.1007/s11120-004-2082-7. Photosynth Res. 2005. PMID: 16143848 Review.
-
Regulation of the assimilation of nitrogen compounds.Annu Rev Biochem. 1978;47:1127-62. doi: 10.1146/annurev.bi.47.070178.005403. Annu Rev Biochem. 1978. PMID: 28074 Review. No abstract available.
Cited by
-
Function and Benefits of Natural Competence in Cyanobacteria: From Ecology to Targeted Manipulation.Life (Basel). 2020 Oct 22;10(11):249. doi: 10.3390/life10110249. Life (Basel). 2020. PMID: 33105681 Free PMC article. Review.
-
Genomics Approaches to Deciphering Natural Transformation in Cyanobacteria.Front Microbiol. 2019 Jun 7;10:1259. doi: 10.3389/fmicb.2019.01259. eCollection 2019. Front Microbiol. 2019. PMID: 31231343 Free PMC article.