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Case Reports
. 2009 Sep;15(9):1478-80.
doi: 10.3201/eid1509.090358.

Human Plasmodium knowlesi infection detected by rapid diagnostic tests for malaria

Jaap J van Hellemond  1 , Marijke Rutten, Rob Koelewijn, Anne Marie Zeeman, Jaco J Verweij, Pieter J Wismans, Clemens H Kocken, Perry J J van Genderen
Affiliations
Case Reports

Human Plasmodium knowlesi infection detected by rapid diagnostic tests for malaria

Jaap J van Hellemond et al. Emerg Infect Dis. 2009 Sep.

Abstract

We describe a PCR-confirmed case of Plasmodium knowlesi infection with a high parasitemia level and clinical signs of severe malaria in a migrant worker from Malaysian Borneo in the Netherlands. Investigations showed that commercially available rapid antigen tests for detection of human Plasmodium infections can detect P. knowlesi infections in humans.

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Figures

Figure 1
Figure 1
Morphology of Plasmodium knowlesi in a Giemsa-stained thin blood smear. Infected erythrocytes were not enlarged, lacked Schuffner stippling, and contained much pigment. Shown are examples of trophozoites (A–F), a schizont (G), and a gametocyte (H). Scale bars = 5 μm.
Figure 2
Figure 2
Phylogenetic tree constructed according to the neighbor-joining method based on A-type small subunit RNA sequences of several Plasmodium species (GenBank accession numbers are indicated). The sequence of the clinical isolate PkHHR-BPRC1 (in boldface) (GenBank accession no. FJ804768) clusters with all other P. knowlesi strains (indicated by Pk isolate numbers). Pfrag, P. fragile; Pinui, P. inui; Pcyn, P. cynomolgi; Pfalc, P. falciparum; Pmal, P. malariae. Scale bar indicates nucleotide substitutions per site.
See this image and copyright information in PMC

References

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