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. 1990 Nov 25;18(22):6531-5.
doi: 10.1093/nar/18.22.6531.

DNA polymorphisms amplified by arbitrary primers are useful as genetic markers

J G Williams  1 , A R Kubelik, K J Livak, J A Rafalski, S V Tingey
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Free PMC article

DNA polymorphisms amplified by arbitrary primers are useful as genetic markers

J G Williams et al. Nucleic Acids Res. .
Free PMC article

Abstract

Molecular genetic maps are commonly constructed by analyzing the segregation of restriction fragment length polymorphisms (RFLPs) among the progeny of a sexual cross. Here we describe a new DNA polymorphism assay based on the amplification of random DNA segments with single primers of arbitrary nucleotide sequence. These polymorphisms, simply detected as DNA segments which amplify from one parent but not the other, are inherited in a Mendelian fashion and can be used to construct genetic maps in a variety of species. We suggest that these polymorphisms be called RAPD markers, after Random Amplified Polymorphic DNA.

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References

    1. Am J Hum Genet. 1980 May;32(3):314-31 - PubMed
    1. Nucleic Acids Res. 1980 Oct 10;8(19):4321-5 - PubMed
    1. Genomics. 1987 Oct;1(2):174-81 - PubMed
    1. Science. 1989 Sep 29;245(4925):1434-5 - PubMed
    1. Science. 1988 Oct 14;242(4876):229-37 - PubMed