Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Dec;83(24):13015-8.
doi: 10.1128/JVI.01714-09. Epub 2009 Sep 30.

Ostrich involvement in the selection of H5N1 influenza virus possessing mammalian-type amino acids in the PB2 protein

Kyoko Shinya  1 , Akiko Makino, Makoto Ozawa, Jin Hyun Kim, Yuko Sakai-Tagawa, Mutsumi Ito, Quynh Mai Le, Yoshihiro Kawaoka
Affiliations

Ostrich involvement in the selection of H5N1 influenza virus possessing mammalian-type amino acids in the PB2 protein

Kyoko Shinya et al. J Virol. 2009 Dec.

Abstract

Amino acids at positions 627 and 701 in the PB2 protein (PB2-627 and PB2-701, respectively) of avian influenza A viruses affect virus replication in some mammalian cells. Highly pathogenic H5N1 influenza viruses possessing mammalian-type PB2-627 were detected during the Qinghai Lake outbreak in 2005 and spread to Europe and Africa. Via a database search, we found a high rate of viral isolates from Ratitae, including ostrich, possessing mammalian-type PB2-627 or -701. Here, we report that H5N1 avian influenza viruses possessing mammalian-type amino acids in PB2-627 or -701 are selected during replication in ostrich cells in vitro and in vivo.

PubMed Disclaimer

Figures

FIG. 1.
FIG. 1.
Virus populations from trachea, lung, and brain samples from infected ostriches, characterized by the amino acid at position 627 in PB2. The percentage of viruses possessing the PB2-627Lys mutation is indicated for each organ.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Allen, J. E., S. N. Gardner, E. A. Vitalis, and T. R. Slezak. 2009. Conserved amino acid markers from past influenza pandemic strains. BMC Microbiol. 9:77. - PMC - PubMed
    1. Basler, C. F., and P. V. Aguilar. 2008. Progress in identifying virulence determinants of the 1918 H1N1 and the Southeast Asian H5N1 influenza A viruses. Antivir. Res. 79:166-178. - PMC - PubMed
    1. Buckler-White, A. J., C. W. Naeve, and B. R. Murphy. 1986. Characterization of a gene coding for M proteins which is involved in host range restriction of an avian influenza A virus in monkeys. J. Virol. 57:697-700. - PMC - PubMed
    1. Chen, G. W., S. C. Chang, C. K. Mok, Y. L. Lo, Y. N. Kung, J. H. Huang, Y. H. Shih, J. Y. Wang, C. Chiang, C. J. Chen, and S. R. Shih. 2006. Genomic signatures of human versus avian influenza A viruses. Emerg. Infect. Dis. 12:1353-1360. - PMC - PubMed
    1. Chen, H., Y. Li, Z. Li, J. Shi, K. Shinya, G. Deng, Q. Qi, G. Tian, S. Fan, H. Zhao, Y. Sun, and Y. Kawaoka. 2006. Properties and dissemination of H5N1 viruses isolated during an influenza outbreak in migratory waterfowl in western China. J. Virol. 80:5976-5983. - PMC - PubMed

Publication types

MeSH terms

Substances