Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2009 Nov;47(11):3747-8.
doi: 10.1128/JCM.01391-09. Epub 2009 Sep 30.

Failure of the BD GeneOhm StaphSR assay for direct detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates in positive blood cultures collected in the United States

James W Snyder  1 , Gina K Munier, Stacy A Heckman, Pamela Camp, Timothy L Overman
Affiliations
Comparative Study

Failure of the BD GeneOhm StaphSR assay for direct detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates in positive blood cultures collected in the United States

James W Snyder et al. J Clin Microbiol. 2009 Nov.

Abstract

Fifty-nine Staphylococcus aureus isolates from one hundred blood cultures containing gram-positive cocci in clusters were identified by conventional methods and the BD GeneOhm StaphSR assay (SR). The SR misidentified three methicillin (meticillin)-resistant S. aureus (MRSA) isolates as methicillin-susceptible S. aureus (MSSA), while one MSSA isolate tested negative for S. aureus. The three MRSA isolates were strains with MREJ types that cannot be detected by the currently available SR.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Bartels, M. D., K. Boye, S. M. Rhode, A. R. Larsen, H. Torfs, P. Bouchy, R. Skov, and H. Westh. 2009. A common variant of staphylococcal cassette chromosome mec type IVa in isolates from Copenhagen, Denmark, is not detected by the BD GeneOhm methicillin-resistant Staphylococcus aureus assay. J. Clin. Microbiol. 47:1524-1527. - PMC - PubMed
    1. Cosgrove, S. E., G. Sakoulas, E. N. Perencevich, M. J. Schwaber, A. W. Karchmer, and Y. Carmeli. 2003. Comparison of mortality associated with methicillin-resistant and methicillin-susceptible Staphylococcus aureus bacteremia: a meta-analysis. Clin. Infect. Dis. 36:53-59. - PubMed
    1. Gröbner, S., M. Dion, M. Plante, and V. A. J. Kempf. 2009. Evaluation of the BD GeneOhm StaphSR assay for detection of methicillin-resistant and methicillin-susceptible Staphylococcus aureus isolates from spiked positive blood cultures. J. Clin. Microbiol. 47:1689-1694. - PMC - PubMed
    1. Huletsky, A., R. Giroux, V. Rossbach, M. Gagnon, M. Vaillancourt, M. Bernier, F. Gagnon, K. Truchon, M. Bastien, F. J. Picard, A. Van Belkum, M. Ouellette, P. H. Roy, and M. G. Bergeron. 2004. New real-time PCR assay for rapid detection of methicillin-resistant Staphylococcus aureus directly from specimens containing a mixture of staphylococci. J. Clin. Microbiol. 42:1875-1884. - PMC - PubMed
    1. Stamper, P. D., M. Cai, T. Howard, S. Speser, and K. C. Carroll. 2007. Clinical validation of the molecular BD GeneOhm StaphSR assay for direct detection of Staphylococcus aureus and methicillin-resistant Staphylococcus aureus in positive blood cultures. J. Clin. Microbiol. 45:2191-2196. - PMC - PubMed

MeSH terms

Substances