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. 2009 Oct;58(10):2168-74.
doi: 10.2337/db09-0318.

Application of isotopic techniques using constant specific activity or enrichment to the study of carbohydrate metabolism

Adrian Vella  1 , Robert A Rizza
Affiliations

Application of isotopic techniques using constant specific activity or enrichment to the study of carbohydrate metabolism

Adrian Vella et al. Diabetes. 2009 Oct.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Schematic diagram illustrating the multiple-pool concept in tracer dilution studies. Pool a represents the readily accessible pool in which tracer distributes rapidly, in contrast to the less readily accessible pool (Pool b). The relative size of each pool can be altered by experimental (or physiological) conditions, as can the flow of tracer (and tracee) from Pool a to b (Vab) and vice versa (Vba). F, tracer infusion rate.
FIG. 2.
FIG. 2.
An experiment using intravenous infusion of glucose to mimic the systemic appearance of oral glucose (A). The tracer [3-3H]glucose is infused via two separate infusions (B), so that tracer infusion 1 mimics the expected decline in EGP. Tracer infusion 2 reflects the glucose infusion replicating systemic appearance of oral glucose. Despite large, rapid changes in glucose concentration, the SA is relatively constant over time (C), enabling accurate measurement of EGP (D).
FIG. 3.
FIG. 3.
Methodology for the determination of splanchnic glucose uptake (SGU) using a nasojejunal tube.
FIG. 4.
FIG. 4.
Calculation of the systemic appearance of meal-derived glucose (A), EGP (B), and glucose disappearance (C) using the triple-tracer technique and a one-compartment model. Note that changing the estimates of the pool fraction (pV) has little effect on calculated rates. In contrast, using a dual-tracer approach, changing estimates of pV have marked effects on meal appearance (D), EGP (E), and glucose disappearance (F). Modified and reprinted with permission from Basu et al. Am J Physiol Endocrinol Metab 2003;284:E55–E69.
See this image and copyright information in PMC

References

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    1. Dinneen S, Gerich J, Rizza R: Carbohydrate metabolism in non-insulin-dependent diabetes mellitus. N Engl J Med 1992;327:707–713 - PubMed
    1. Wolfe RR, Chinkes DL: Isotope Tracers in Metabolic Research: Principles and Practice of Kinetic Analysis 2nd ed.Hoboken, NJ, Wiley-Liss, 2005
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    1. McMahon MM, Schwenk WF, Haymond MW, Rizza RA: Underestimation of glucose turnover measured with [6–3H]- and [6,6–2H]- but not [6–14C]glucose during hyperinsulinemia in humans. Diabetes 1989;38:97–107 - PubMed

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