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. 2009 Sep;45(2):241-7.
doi: 10.3164/jcbn.09-42. Epub 2009 Aug 28.

Effects of Lansoprazole on the Lipopolysaccharide-Stimulated Toll-Like Receptor 4 Signal Transduction Systems: A Study Using the 293hTLR4/MD2-CD14 Cells

Tadashi Ohara  1 , Yuhsaku Kanoh, Keiichi Yoshino, Masaki Kitajima
Affiliations

Effects of Lansoprazole on the Lipopolysaccharide-Stimulated Toll-Like Receptor 4 Signal Transduction Systems: A Study Using the 293hTLR4/MD2-CD14 Cells

Tadashi Ohara et al. J Clin Biochem Nutr. 2009 Sep.

Abstract

The present study was undertaken to evaluate the effects of lansoprazole (LPZ) on lipopolysaccharide (LPS)-stimulated toll-like receptor 4 (TLR4) signal transduction systems using the 293hTLR4/MD2-CD14 cells. The cells were incubated and then divided into the following groups: (a) untreated group, (b) non-LPZ treated (1h) group, (c) LPZ-treated (1h) plus non LPS-stimulated (1h) group, (d) LPZ-treated (1h) plus non LPS-stimulated (6h) group, (e) LPZ-treated (1h) plus LPS-stimulated (1h) group, (f) LPZ-treated (1h) plus LPS-stimulated (6h) group, (g) non LPZ-treated (1h) plus LPS-stimulated (1h) group and (h) non LPZ-treated (1h) plus LPS-stimulated (6h) group. Samples from each group were subjected to western blotting for analysis of IkB phosphorylation, intranuclear transfer of NF-kB, phosphorylation of MAP kinase (MAPK), intranuclear transfer of interferon regulatory factor 5 (IRF5), and expression of suppressor of cytokine signaling-1 (SOCS1). In the LPZ-treated groups, neither phosphorylation of MAPK nor intranuclear transfer of IRF5 was suppressed under stimulation with LPS, and enhanced intranuclear transfer of NF-kB and increased expression of SOCS1 were noted by comparison with the group treated with LPS alone. These results suggest that LPZ stimulates the expression of SOCS1 and regulates protein phosphorylation through its activity on TLR4 signal transduction under LPS stimulation.

Keywords: 293hTLR4/MD2-CD14 cell; SOCS1; TLR4; lansoprazole; lipopolysaccharide.

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Figures

Fig. 1
Fig. 1
The phosphorylation of IkB-α analyzed by western-blotting. An arrow indicates the induction of IkB-α. LPZ and LPS denotes lansoprazole and lipopolysaccharide, respectively.
Fig. 2
Fig. 2
The phosphorylation of ERK (phospho-p44/42 MAPK antibody and p44/42 MAPK antibody) analyzed by western-blotting. Arrows indicate the induction of phospho-p44/42 MAPK antibody and p44/42 MAPK antibody, respectively.
Fig. 3
Fig. 3
The phosphorylation of JNK (phospho-SAPK/JNK (Thr183/Tyr185) antibody and SAPK/JNK (56G8) rabbit mAb) analyzed by western-blotting. Arrows indicate the induction of phospho-SAPK/JNK (Thr183/Tyr185) antibody. The phosphorylation of SAPK/JNK (56G8) rabbit mAb was obscure.
Fig. 4
Fig. 4
The phosphorylation of p38 (phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody) analyzed by western-blotting. An arrow indicates the induction of phospho-p38MAPK (Thr180/Tyr182) antibody and p38MAPK antibody, respectively.
Fig. 5
Fig. 5
The result of the transition from cytoplasm to nucleus of NF-kBp65 analyzed by western-blotting.An arrow indicates the induction of NF-kB.
Fig. 6
Fig. 6
The result of the transition from cytoplasm to nucleus of IRF5 analyzed by western-blotting. An arrow indicates the induction of IRF5.
Fig. 7
Fig. 7
The expression of SOCS1 analyzed by western-blotting. An arrow indicates the induction of SOCS1.
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References

    1. Akira S., Takeda K., Kaisho T. Toll-like receptors: critical proteins linking innate and acquired immunity. Nat. Immunol. 2001;2:675–680. - PubMed
    1. Takeuchi O., Hoshino K., Akira S. TLR 2-deficient and MyD88-deficient mice are highly susceptible to Staphylococcus aureus infection. J. Immunol. 2000;165:5392–5396. - PubMed
    1. Diebold S.S. Activation of dendritic cells by toll-like receptors and C-type lectins. Handb. Exp. Pharmacol. 2009;188:3–30. - PubMed
    1. Goodridge F.S., Shimada T., Wolf A.J., Hsu Y.M., Becker C.A., Lin X., Underhill D.M. Differential use of CARD9 by Dectin-1 in macrophages and dendritic cells. J. Immunol. 2009;182:1146–1154. - PMC - PubMed
    1. Beutler B. Microbe sensing, positive feedback loops, and the pathogenesis of inflammatory diseases. Immunol. Rev. 2009;227:248–263. - PMC - PubMed