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. 1990 Nov;35(6):1277-83.

Effects of cytosine methylation at restriction sites on deoxyribonucleic acid (DNA) typing

K Washio  1 , S Ueda, S Misawa
Affiliations
  • PMID: 1979803

Effects of cytosine methylation at restriction sites on deoxyribonucleic acid (DNA) typing

K Washio et al. J Forensic Sci. 1990 Nov.

Abstract

The effects of endogenous 5-methylcytosines on deoxyribonucleic acid (DNA) fingerprints were studied. Analysis with methylation-sensitive restriction endonuclease Sau3AI and its methylation-insensitive isoschizomer MboI showed some differences in the patterns generated as a result of 5-methylcytosines at the recognition sites. Moreover, a few bands of sperm DNA did not match those of blood DNA from the same individual, a phenomenon only observed in the digests of methylation-sensitive endonucleases. These findings indicate the unsuitability of methylation-sensitive restriction endonucleases for DNA fingerprinting and other forms of DNA typing, because of the tissue-specific status of the methylation.

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