Genome-wide high-resolution chromatin immunoprecipitation of meiotic chromosomal proteins in Saccharomyces cerevisiae

Abstract

Cooperative actions of chromosomal proteins play critical roles in the dynamics, structural transition, segregation, and maintenance of meiotic chromosomes. A high-resolutibn genome-tiling array combined with a chromatin immunoprecipitation assay (ChIP-chip) is a powerful tool for uncovering the precise and dynamic distributions of various proteins along meiotic chromosomes. In this chapter, we describe a method to map the binding sites of meiotic chromosomal proteins such as Spo11, Mre11, and Rec8 using the high-resolution ChIP-chip technology. This system provides us with informative knowledge on the interplay of meiotic chromosomal proteins.