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. 2009 Nov;75(22):7271-4.
doi: 10.1128/AEM.00687-09. Epub 2009 Oct 2.

Occurrence of putative pathogenicity islands in enterococci from distinct species and of differing origins

Teresa Semedo-Lemsaddek  1 , Maria Teresa Barreto-Crespo, Rogério Tenreiro
Affiliations

Occurrence of putative pathogenicity islands in enterococci from distinct species and of differing origins

Teresa Semedo-Lemsaddek et al. Appl Environ Microbiol. 2009 Nov.

Abstract

Enterococci isolated from ewe's milk and cheese, clinical isolates of human and veterinary origins, and reference strains obtained from culture collections were screened for the occurrence of putative pathogenicity island (PAIs). Results obtained after PCR amplification and hybridization point toward PAI dissemination among enterococci of diverse origins (food/clinical) and species (Enterococcus faecalis/non-E. faecalis).

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Figures

FIG. 1.
FIG. 1.
Genetic organization of the putative enterococcal PAIs (dendrogram obtained with the BioNumerics software, version 4.61 [Applied Maths, Kortrijk, Belgium], Pearson's correlation coefficient, and the unweighted-pair group method using average linkages). Gray fill represents positive amplification of the corresponding PAI region (PAI-harboring E. faecalis MMH594 is in black for comparison). The numbers 1 to 33 correspond to the regions or loci in Table S2 in the supplemental material.
FIG. 2.
FIG. 2.
Macrorestriction profile obtained with the endonuclease I-Ceu-I (left panel) and corresponding hybridization with 16S rRNA gene (center panel) and cylMBA (right panel) probes. Lanes 1 and 18, λ ladder PFGE marker (New England BioLabs); lane 2, DSMZ 20680T (the superscript T indicates the type strain); lane 3, DSMZ 5633T; lane 4, V583; lane 5, MMH594; lane 6, E300; lane 7, H1881; lane 8, V95; lane 9, V434; lane 10, LN9; lane 11, LN11; lane 12, QSE123; lane 13, LA78; lane 14, LA160; lane 15, QA29a; lane 16, QA40; lane 17, QCB54.
FIG. 3.
FIG. 3.
Hybridization signals obtained by using the PAI-related genes esp (left panel) and cylMBA (right panel) as probes. Left panel: lanes 1 and 17, λ ladder PFGE marker (New England BioLabs); lanes 2 to 8, MMH594, E300, V434, LN9, LN11, LA78, and QA40 cleaved with SfiI; lanes 9 to 16, V583, E300, V434, LN9, LN11, LA78, QA40, and QCB54 cleaved with SmaI. Right panel: lanes 1 and 19, λ ladder PFGE marker (New England BioLabs); lanes 2 to 10, MMH594, E300, V434, LN9, LN11, LA78, QA40, QCB 54, and V583 cleaved with SfiI; lanes 11 to 18, MMH594, E300, V434, LN9, LN11, LA78, QA40, and QCB54 cleaved with SmaI.
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