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. 2009 Sep;71(9):1217-20.
doi: 10.1292/jvms.71.1217.

Conservation of the Sapsaree (Canis familiaris), a Korean Natural Monument, using somatic cell nuclear transfer

Goo Jang  1 , SoGun Hong, JungTaek Kang, JungEun Park, HyunJu Oh, ChanKyu Park, JiHong Ha, DaeYong Kim, MinKyu Kim, ByeongChun Lee
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Free article

Conservation of the Sapsaree (Canis familiaris), a Korean Natural Monument, using somatic cell nuclear transfer

Goo Jang et al. J Vet Med Sci. 2009 Sep.
Free article

Abstract

A recent emerging technology, somatic cell nuclear transfer (SCNT), has been considered for conserving threatened or endangered species. Sapsaree is a native breed in Korea and has been designated as a Natural Monument. The aim of this study was to produce a Sapsaree by SCNT for breed conservation. Donor fibroblasts from a 9-year-old male Sapsaree were placed into the perivitelline spaces of enucleated in vivo matured oocytes and fused electrically. A total of 309 cloned embryos were transferred into the oviducts of 15 naturally synchronized recipients. Two recipients were diagnosed as pregnant, and each delivered one cloned puppy, both of which weighed 530 g. Overall, this study demonstrated that an endangered canine breed can be conserved by SCNT.

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