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. 2009 Nov;108(5):435-7.
doi: 10.1016/j.jbiosc.2009.05.013.

Effects of siRNAs targeting the spacer sequence of plant RNAi vectors on the specificity and efficiency of RNAi

Kazuma Nishimura  1 , Sayaka Hirai, Hiroaki Kodama
Affiliations

Effects of siRNAs targeting the spacer sequence of plant RNAi vectors on the specificity and efficiency of RNAi

Kazuma Nishimura et al. J Biosci Bioeng. 2009 Nov.

Abstract

Small interfering RNAs (siRNAs) generated from the spacer region of hairpin RNAs were not detected in the RNA interference (RNAi) plants targeting the fatty acid desaturase gene. The expression of the desaturase gene was stably suppressed even when siRNAs targeting the spacer sequences were introduced into this plant.

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