Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 May;1(6):465-9.
doi: 10.1091/mbc.1.6.465.

Activation by serotonin of starfish eggs expressing the rat serotonin 1c receptor

Affiliations

Activation by serotonin of starfish eggs expressing the rat serotonin 1c receptor

F Shilling et al. Cell Regul. 1990 May.

Abstract

Starfish oocytes were injected with mRNA for the serotonin 1c receptor or with rat brain poly A+ mRNA, incubated to allow expression of the membrane protein, then matured to eggs by addition of 1-methyladenine. Applying serotonin to these eggs caused cortical granule exocytosis like that occurring at fertilization. Because the serotonin 1c receptor specifically activates a G-protein, these results provide support for the hypothesis that sperm activate eggs by way of a receptor-G-protein interaction. The starfish oocyte may be a generally useful system for expression of exogenous mRNA for membrane proteins.

PubMed Disclaimer

References

    1. Dev Biol. 1987 Apr;120(2):577-83 - PubMed
    1. Methods Cell Biol. 1986;27:379-94 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Dec;84(23):8721-5 - PubMed
    1. Brain Res. 1987 Dec;427(1):83-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Oct;85(19):7260-4 - PubMed

Publication types

LinkOut - more resources