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. 1990 Oct;36(4):149-55.
doi: 10.1111/j.1399-0039.1990.tb01821.x.

The application of oligonucleotide probes to HLA class II typing of the DRB sub-region

R W Vaughan  1 , J S Lanchbury, S G Marsh, M A Hall, J G Bodmer, K I Welsh
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The application of oligonucleotide probes to HLA class II typing of the DRB sub-region

R W Vaughan et al. Tissue Antigens. 1990 Oct.

Abstract

A plan for DRB typing at the sequence level is detailed. Only one polymerase chain amplification reaction is needed and the application of a limited number of short oligonucleotide probes allows an almost complete definition of DRB alleles. The scheme was tested on 40 homozygous cell-lines selected to cover a wide range of specificities, and 40 RFLP-typed controls. The results are presented and discussed. The simplicity and accuracy of this scheme are emphasized.

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