Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2009 Jul;4(7):682-4.
doi: 10.4161/psb.4.7.9078. Epub 2009 Jul 20.

EHD2 inhibits signaling of leucine rich repeat receptor-like proteins

Maya Bar  1 , Adi Avni
Affiliations
Comment

EHD2 inhibits signaling of leucine rich repeat receptor-like proteins

Maya Bar et al. Plant Signal Behav. 2009 Jul.

Abstract

Leucine-rich-repeat receptor protein (LRR-RLPs) and LRR-RLKs have been linked with signaling and defense responses in plants. EIX (ethylene-inducing xylanase) triggers a defense response via the LeEix2 receptor, while bacterial flagellin triggers plant innate immunity via the FLS2 receptor. Endocytosis has been suggested to be crucial for the process in both cases. Recently, we showed that the EIX elicitor triggers internalization of the LeEix2 receptor. Additionally, we demonstrate that plant EHD2 is an important factor in the internalization and regulation of the induction of plant immunity in the case of EIX/LeEix2 but does not appear to be involved in the flg/FLS2 system. Here we show that EHD2 is also involved in the signaling of the Cf4 and Cf9 receptors, causing inhibition of hypersensitive response (HR) and ethylene biosynthesis upon overexpression of EHD2. Our results suggest that different endocytosis pathways are involved in the induction of plant defense responses.

PubMed Disclaimer

Figures

Figure 1
Figure 1
EHD2 overexpression inhibits HR induction and ethylene biosynthesis. (A) N. tabacum transiently transformed with Pro35S:tvEIX or a mixture of Pro35S:Cf9 and Pro35S:Avr9 or Pro35S:Cf4 and Pro35S:Avr4 and either Pro35S:AtEHD2 or Pro35S:GFP. Induction of HR was monitored 96 h after transformation. (B) Leaf disks of transiently transformed tobacco leaves with either mock (Pro35S:GFP) or Pro35S:AtEHD2 and Pro35S:Cf9/Pro35S:Avr9 (48 h after transformation), were floated on a 250 mM Sorbitol solution; Leaf disks of transiently transformed tobacco leaves with either mock (Pro35S:GFP) or Pro35S:AtEHD2 were also floated on a 250 mM Sorbitol solution supplemented with 2.5 µg/ml EIX. Ethylene biosynthesis was measured after 4 hours. Error bars represent the average ± SE of 4 different experiments.
Figure 2
Figure 2
Schematic representation of defense responses mediated by FLS2 (LRR-RLK; left) and LeEix2 (LRR-RLP; right). FLS2 mediates e.g., ROS production, Ethylene biosynthesis and PR protein synthesis via its kinase domain in response to flagellin (flg22). LeEix2 interacts with EHD2 and possibly AP-2 and mediates e.g., ROS production, Ethylene biosynthesis, PR protein synthesis, EHD2 expression and HR in response to EIX.
See this image and copyright information in PMC

Comment on

References

    1. Torii KU. Leucine-rich repeat receptor kinases in plants: structure, function and signal transduction pathways. Int Rev Cytol. 2004;234:1–46. - PubMed
    1. Felix G, Duran JD, Volko S, Boller T. Plants have a sensitive perception system for the most conserved domain of bacterial flagellin. Plant Journal. 1999;18:265–276. - PubMed
    1. Gomez-Gomez L, Boller T. FLS2: An LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis. Mol Cell. 2000;5:1003–1011. - PubMed
    1. Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, et al. MAP kinase signaling cascade in Arabidopsis innate immunity. Nature. 2002;415:977–983. - PubMed
    1. Zipfel C, Robatzek S, Navarro L, Oakeley EJ, Jones JDG, Felix G, et al. Bacterial disease resistance in Arabidopsis through flagellin perception. Nature. 2004;428:764–767. - PubMed

LinkOut - more resources