One year experience on the Lindholm B medium used in large--scale FMD virus production on BHK cells in suspension

Abstract

The propagation of FMD virus on BHK 21 clone 13 suspension cells was based on the Lindholm B medium during the production period August 1975 to June 1976. The medium was used in connection with a production cycle including decreasing amounts of serum in the cell growth medium and inoculation of the cells while they were still in growth. This method of virus production, compared with the traditional method, is cheaper due to the lowered medium cost. The improved utilization of the fermenter capacity and the decreased consumption of serum have practical as well as economic aspects. The results obtained during the period indicate that virus production by this method is reliable and at least on the same level as by traditional methods.