Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Nov;6(11):809-11.
doi: 10.1038/nmeth.1392. Epub 2009 Oct 18.

Enrichment of glycopeptides for glycan structure and attachment site identification

Jonas Nilsson  1 , Ulla Rüetschi, Adnan Halim, Camilla Hesse, Elisabet Carlsohn, Gunnar Brinkmalm, Göran Larson
Affiliations

Enrichment of glycopeptides for glycan structure and attachment site identification

Jonas Nilsson et al. Nat Methods. 2009 Nov.

Abstract

We present a method to enrich for glycoproteins from proteomic samples. Sialylated glycoproteins were selectively periodate-oxidized, captured on hydrazide beads, trypsinized and released by acid hydrolysis of sialic acid glycosidic bonds. Mass spectrometric fragment analysis allowed identification of glycan structures, and additional fragmentation of deglycosylated ions yielded peptide sequence information, which allowed glycan attachment site and protein identification. We identified 36 N-linked and 44 O-linked glycosylation sites on glycoproteins from human cerebrospinal fluid.

PubMed Disclaimer

References

    1. J Biol Chem. 2003 Jan 3;278(1):573-84 - PubMed
    1. Nat Med. 2008 Jun;14(6):648-55 - PubMed
    1. Rapid Commun Mass Spectrom. 2004;18(24):2983-8 - PubMed
    1. Rapid Commun Mass Spectrom. 2008;22(6):905-8 - PubMed
    1. Cell. 2006 Sep 8;126(5):855-67 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources