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. 2009;48(47):8970-3.
doi: 10.1002/anie.200901668.

Three-dimensional nanostructured substrates toward efficient capture of circulating tumor cells

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Three-dimensional nanostructured substrates toward efficient capture of circulating tumor cells

Shutao Wang et al. Angew Chem Int Ed Engl. 2009.
No abstract available

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Figures

Figure 1
Figure 1
Conceptual illustration of how an anti-EpCAM-coated 3D nanostructured (i.e., SiNP) substrate can be employed to achieve significantly enhanced capture of EpCAM-positive cells (i.e., CTCs) from cell suspension in contrast to an anti-EpCAM-coated unstructured (i.e., flat Si) substrate. a) Interdigitation of nanoscale cellular surface components and SiNPs enhances local topographic interactions, resulting in vastly improved cell-capture efficiency. b) Lack of local topographic interactions between cells and flat Si substrate compromises the respective cell-capture efficiency.
Figure 2
Figure 2
a) Fluorescence micrographs and SEM images of SiNP substrates and flat Si substrates on which MCF7 cells were captured. The SiNP substrates exhibited significantly better cell-capture efficiency than the flat ones. DAPI: 4’,6-Diamidino-2-phenylindol. b) Photolithography process for patterning alternate SiNP and flat substrates on the silicon wafer for comparing their cell-capture efficiencies in a close experimental setup. c) SEM images of patterned and flat substrates before cell capture (top) and fluorescence micrographs of cells captured on patterned and flat substrates (bottom).
Figure 3
Figure 3
Quantitative evaluations of cell-capture yields a) at different capture times and b) with different SiNP lengths ranging from 0 to 20 mm. Each plot and error bar represents a mean standard deviation from three repeats.
Scheme 1
Scheme 1
A) Chemical etching by Ag+ and HF was employed to produce a silicon nanopillar (SiNP) array on a silicon wafer. The SEM images reveal that well-defined SiNPs with diameters ranging from 100 to 200 nm and lengths around 10 µm were produced. B) Grafting of biotinylated epithelial-cell adhesion-molecule antibody (anti-EpCAM) onto silicon substrates.

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