Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009;48(47):8970-3.
doi: 10.1002/anie.200901668.

Three-dimensional nanostructured substrates toward efficient capture of circulating tumor cells

Shutao Wang  1 , Hao Wang, Jing Jiao, Kuan-Ju Chen, Gwen E Owens, Ken-ichiro Kamei, Jing Sun, David J Sherman, Christian P Behrenbruch, Hong Wu, Hsian-Rong Tseng
Affiliations

Three-dimensional nanostructured substrates toward efficient capture of circulating tumor cells

Shutao Wang et al. Angew Chem Int Ed Engl. 2009.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Conceptual illustration of how an anti-EpCAM-coated 3D nanostructured (i.e., SiNP) substrate can be employed to achieve significantly enhanced capture of EpCAM-positive cells (i.e., CTCs) from cell suspension in contrast to an anti-EpCAM-coated unstructured (i.e., flat Si) substrate. a) Interdigitation of nanoscale cellular surface components and SiNPs enhances local topographic interactions, resulting in vastly improved cell-capture efficiency. b) Lack of local topographic interactions between cells and flat Si substrate compromises the respective cell-capture efficiency.
Figure 2
Figure 2
a) Fluorescence micrographs and SEM images of SiNP substrates and flat Si substrates on which MCF7 cells were captured. The SiNP substrates exhibited significantly better cell-capture efficiency than the flat ones. DAPI: 4’,6-Diamidino-2-phenylindol. b) Photolithography process for patterning alternate SiNP and flat substrates on the silicon wafer for comparing their cell-capture efficiencies in a close experimental setup. c) SEM images of patterned and flat substrates before cell capture (top) and fluorescence micrographs of cells captured on patterned and flat substrates (bottom).
Figure 3
Figure 3
Quantitative evaluations of cell-capture yields a) at different capture times and b) with different SiNP lengths ranging from 0 to 20 mm. Each plot and error bar represents a mean standard deviation from three repeats.
Scheme 1
Scheme 1
A) Chemical etching by Ag+ and HF was employed to produce a silicon nanopillar (SiNP) array on a silicon wafer. The SEM images reveal that well-defined SiNPs with diameters ranging from 100 to 200 nm and lengths around 10 µm were produced. B) Grafting of biotinylated epithelial-cell adhesion-molecule antibody (anti-EpCAM) onto silicon substrates.
See this image and copyright information in PMC

References

    1. Steeg PS. Nat. Med. 2006;12:895. - PubMed
    1. Budd GT, Cristofanilli M, Ellis MJ, Stopeck A, Borden E, Miller MC, Matera J, Repollet M, Doyle GV, Terstappen LWMM, Hayes DF. Clin. Cancer Res. 2006;12:6403. - PubMed
    1. Allard WJ, Matera J, Miller MC, Repollet M, Connelly MC, Rao C, Tibbe AGJ, Uhr JW, Terstappen LWMM. Clin. Cancer Res. 2004;10:6897. - PubMed
    1. Zieglschmid V, Hollmann C, Böcher O. Crit. Rev. Clin. Lab. Sci. 2005;42:155. - PubMed
    1. Pantel K, Brakenhoff RH, Brandt B. Nat. Rev. Cancer. 2008;8:329. - PubMed

Publication types

MeSH terms

LinkOut - more resources