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Comment
. 2010 Jan;192(1):26-8.
doi: 10.1128/JB.01316-09.

Global approaches for finding small RNA and small open reading frame functions

Karen M Wassarman  1 , Patricia J Kiley
Affiliations
Comment

Global approaches for finding small RNA and small open reading frame functions

Karen M Wassarman et al. J Bacteriol. 2010 Jan.
No abstract available

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Figures

FIG. 1.
FIG. 1.
Schematic representation of a bar code and the regions that will be amplified and detected for analysis (A) and the regions amplified from bar codes from different mutants (B). UP and DN are the unique sequences that will be different for every mutation generated. com1, com2, and com3 are common sequences that are the same sequence for every mutation made and serve as priming sites. Primers are indicated by black arrows above the bar codes (1a, 1b, 2a, and 2b). Note that the com2 sequence from the Hobbs et al. report contains a gene to confer kanamycin resistance to facilitate mutant strain generation, but drug resistance is not used for selection when screening mutant libraries for phenotypes. In some cases, the kanamycin resistance gene was removed to prevent any polar effects, but primer sites 1b and 2a were retained. In panel B, each UP and DN bar code represented comes from a different strain, as indicated by the change in color of the unique UP or DN sequence.
See this image and copyright information in PMC

Comment on

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