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. 1991 Jan;11(1):213-7.
doi: 10.1128/mcb.11.1.213-217.1991.

A chicken beta-actin gene can complement a disruption of the Saccharomyces cerevisiae ACT1 gene

R Karlsson  1 , P Aspenström, A S Byström
Affiliations

A chicken beta-actin gene can complement a disruption of the Saccharomyces cerevisiae ACT1 gene

R Karlsson et al. Mol Cell Biol. 1991 Jan.

Abstract

Recently it was demonstrated that beta-actin can be produced in Saccharomyces cerevisiae by using the expression plasmid pY beta actin (R. Karlsson, Gene 68:249-258, 1988), and several site-specific mutants are now being produced in a protein engineering study. To establish a system with which recombinant actin mutants can be tested in vivo and thus enable a correlation to be made with functional effects observed in vitro, a yeast strain lacking endogenous yeast actin and expressing exclusively beta-actin was constructed. This strain is viable but has an altered morphology and a slow-growth phenotype and is temperature sensitive to the point of lethality at 37 degrees C.

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References

    1. Cell. 1985 Jan;40(1):19-26 - PubMed
    1. Nucleic Acids Res. 1983 Dec 10;11(23):8287-301 - PubMed
    1. Mol Cell Biol. 1985 Oct;5(10):2720-32 - PubMed
    1. Annu Rev Biochem. 1986;55:987-1035 - PubMed
    1. Biochemistry. 1986 Oct 7;25(20):6186-92 - PubMed

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