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. 1991 Jan;11(1):564-7.
doi: 10.1128/mcb.11.1.564-567.1991.

Proline-independent binding of PUT3 transcriptional activator protein detected by footprinting in vivo

J D Axelrod  1 , J Majors, M C Brandriss
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Proline-independent binding of PUT3 transcriptional activator protein detected by footprinting in vivo

J D Axelrod et al. Mol Cell Biol. 1991 Jan.

Abstract

The PUT3 gene product is a transcriptional activator required for expression of the enzymes of the proline utilization pathway. Using two methods of footprinting in vivo, we have determined that PUT3 protein is poised at the promoters of the genes encoding these enzymes and that proline-mediated induction modulates the activity of constitutively bound PUT3.

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