Alternative initiation of translation determines cytoplasmic or nuclear localization of basic fibroblast growth factor
- PMID: 1986249
- PMCID: PMC359672
- DOI: 10.1128/mcb.11.1.573-577.1991
Alternative initiation of translation determines cytoplasmic or nuclear localization of basic fibroblast growth factor
Abstract
Three forms of basic fibroblast growth factor (bFGF), initiated at an AUG (18 kDa) and two CUG (21 and 22.5 kDa) start codons, were produced following transfection of COS cells with human hepatoma bFGF cDNA. The subcellular localization of the different forms was investigated directly or by using chimeric genes constructed by fusion of the bFGF and chloramphenicol acetyltransferase open reading frames. The AUG-initiated proteins were cytoplasmic, while the CUG-initiated forms were nuclear. The signal sequence responsible for the nuclear localization of bFGF is contained within 37 amino acid residues between the second CUG and the AUG start codons. Alternative initiation of translation regulates the subcellular localization of bFGF and thus could modulate its role in cell growth and differentiation control.
Similar articles
-
Over-expression of CUG- or AUG-initiated forms of basic fibroblast growth factor in cardiac myocytes results in similar effects on mitosis and protein synthesis but distinct nuclear morphologies.J Mol Cell Cardiol. 1994 Aug;26(8):1045-60. doi: 10.1006/jmcc.1994.1125. J Mol Cell Cardiol. 1994. PMID: 7799445
-
Three forms of rat basic fibroblast growth factor are made from a single mRNA and localize to the nucleus.J Cell Physiol. 1991 Aug;148(2):202-10. doi: 10.1002/jcp.1041480204. J Cell Physiol. 1991. PMID: 1880150
-
Involvement of basic fibroblast growth factor NH2 terminus in nuclear accumulation.Growth Factors. 1994;11(3):163-74. doi: 10.3109/08977199409046914. Growth Factors. 1994. PMID: 7734142
-
High molecular mass forms of basic fibroblast growth factor are initiated by alternative CUG codons.Proc Natl Acad Sci U S A. 1989 Mar;86(6):1836-40. doi: 10.1073/pnas.86.6.1836. Proc Natl Acad Sci U S A. 1989. PMID: 2538817 Free PMC article.
-
Basic fibroblast growth factor (FGF-2): the high molecular weight forms come of age.J Cell Biochem. 2007 Apr 1;100(5):1100-8. doi: 10.1002/jcb.21116. J Cell Biochem. 2007. PMID: 17131363 Review.
Cited by
-
A single mRNA, transcribed from an alternative, erythroid-specific, promoter, codes for two non-myristylated forms of NADH-cytochrome b5 reductase.J Cell Biol. 1992 Jun;117(5):975-86. doi: 10.1083/jcb.117.5.975. J Cell Biol. 1992. PMID: 1577871 Free PMC article.
-
Internal ribosome entry site structural motifs conserved among mammalian fibroblast growth factor 1 alternatively spliced mRNAs.Mol Cell Biol. 2004 Sep;24(17):7622-35. doi: 10.1128/MCB.24.17.7622-7635.2004. Mol Cell Biol. 2004. PMID: 15314170 Free PMC article.
-
Direct effect of basic fibroblast growth factor on gene transcription in a cell-free system.Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5216-20. doi: 10.1073/pnas.89.12.5216. Proc Natl Acad Sci U S A. 1992. PMID: 1535155 Free PMC article.
-
Ribosome queuing enables non-AUG translation to be resistant to multiple protein synthesis inhibitors.Genes Dev. 2019 Jul 1;33(13-14):871-885. doi: 10.1101/gad.324715.119. Epub 2019 Jun 6. Genes Dev. 2019. PMID: 31171704 Free PMC article.
-
Fibroblast growth factor (FGF) homologous factors: new members of the FGF family implicated in nervous system development.Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9850-7. doi: 10.1073/pnas.93.18.9850. Proc Natl Acad Sci U S A. 1996. PMID: 8790420 Free PMC article.
References
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
