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. 2009 Sep 4;10(9):3900-3917.
doi: 10.3390/ijms10093900.

Expression of CD40 and CD40L in gastric cancer tissue and its clinical significance

Rui Li  1 , Wei-Chang Chen  1 , Xue-Qin Pang  1 , Chen Hua  2 , Ling Li  1 , Xue-Guang Zhang  3
Affiliations

Expression of CD40 and CD40L in gastric cancer tissue and its clinical significance

Rui Li et al. Int J Mol Sci. .

Abstract

To study expression of CD40 and CD40L in gastric cancer tissue we assessed gastric cancer patients admitted to the Department of Gastroenterology of The First Affiliated Hospital of Soochow University and control subjects. Gastric cancer and normal (from around tumours) tissue samples were obtained from patients. Venous blood samples (gastric cancer and ulcer groups) were drawn on the morning of the day before surgery for the measurement of peripheral sCD40L. The expression of CD40 in gastric carcinoma specimens was examined immuno-histochemically. The clinicopathological factors, including age, sex, tumor size, gross appearance, degree of cellular differentiation, histological classification, depth of tumor invasion, lymph node metastasis, peritoneal dissemination, and TNM stage were analyzed according to the different expression of CD40. The results indicated a high CD40 expression in gastric cancer tissues. This positive expression of CD40 revealed a significant (P < 0.05) correlation with lymphatic metastasis and tumor TNM stage in gastric cancer patients. It is concluded that higher CD40 expression existed in expanding type tumors and could play an important role in clinical diagnosis of gastric cancer patients.

Keywords: CD138; CD40; CD40L; apoptosis; gastric cancer.

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Figures

Figure 1.
Figure 1.
A. Strong positive expression of CD40 in gastric cancer tissues; B. Negative expression of CD40 in normal tissues around tumor. C. Weak positive expression of CD138 in gastric cancer tissues; D. Positive expression of CD138 in normal tissues around tumor; E. Negative expression of Gelsolin in gastric cancer tissues; F. Strong positive expression of Gelsolin in normal tissues around tumor; G. Positive expression of P53 in gastric cancer tissues; H. Negative expression of P53 in normal tissues around tumor; I. Positive expression of P65 in gastric cancer tissues; J. Negative expression of P65 in normal tissues around tumor.
Figure 1.
Figure 1.
A. Strong positive expression of CD40 in gastric cancer tissues; B. Negative expression of CD40 in normal tissues around tumor. C. Weak positive expression of CD138 in gastric cancer tissues; D. Positive expression of CD138 in normal tissues around tumor; E. Negative expression of Gelsolin in gastric cancer tissues; F. Strong positive expression of Gelsolin in normal tissues around tumor; G. Positive expression of P53 in gastric cancer tissues; H. Negative expression of P53 in normal tissues around tumor; I. Positive expression of P65 in gastric cancer tissues; J. Negative expression of P65 in normal tissues around tumor.
Figure 2.
Figure 2.
Correlation of CD40 expression with lymphatic metastasis.
Figure 3.
Figure 3.
Correlation of CD40 expression with tumor TNM stage.
Figure 4.
Figure 4.
Correlation of CD40 expression with apoptotic index (AI) in gastric cancer tissues, *P < 0.05.
Figure 5.
Figure 5.
sCD40L levels in three different groups, values are mean ±S.D. *P < 0.05.
Figure 6.
Figure 6.
sCD40L levels in before and after surgery, values are mean ± S.D. *P < 0.05.
See this image and copyright information in PMC

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