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. 1962 Dec 1;15(3):579-88.
doi: 10.1083/jcb.15.3.579.

N-VINYLPYRROLIDONE AS A WATER COMPATIBLE CONSTITUENT OF EMBEDDING RESINS FOR SECTIONING IN ELECTRON MICROSCOPY

A C Fabergé  1 , C W Lewis
Affiliations

N-VINYLPYRROLIDONE AS A WATER COMPATIBLE CONSTITUENT OF EMBEDDING RESINS FOR SECTIONING IN ELECTRON MICROSCOPY

A C Fabergé et al. J Cell Biol. .

Abstract

The use as an embedding resin for ultrathin sectioning of a cross-linked triple copolymer of N-vinylpyrrolidone, acrylonitrile, and ethylene glycol dimethacrylate is described. The first of these components is miscible with water, in all proportions, and can be used as a dehydrating agent, or, alternatively, ethanol may be used in the standard way. Polymerization is carried out at 37 degrees C or even lower temperatures. This resin is unsuitable for use after osmium fixatives, but after permanganates it gives results similar to epoxy. Photographs of rye root-tip cells fixed in permanganate and sectioned in this resin are presented. Because of the water-permeable nature of the product and low polymerization temperature, this resin appears to have possibilities for histochemistry.

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References

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    1. Nature. 1959 Aug 1;184(Suppl 6):375-6 - PubMed