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. 1916 Apr 1;23(4):549-55.
doi: 10.1084/jem.23.4.549.

A METHOD FOR OBTAINING SUSPENSIONS OF LIVING CELLS FROM THE FIXED TISSUES, AND FOR THE PLATING OUT OF INDIVIDUAL CELLS

P Rous  1 , F S Jones
Affiliations

A METHOD FOR OBTAINING SUSPENSIONS OF LIVING CELLS FROM THE FIXED TISSUES, AND FOR THE PLATING OUT OF INDIVIDUAL CELLS

P Rous et al. J Exp Med. .

Abstract

Individual, living, tissue cells can be obtained in suspension by digesting with trypsin the clot of growing tissue cultures. Under these circumstances the living cells assume a spherical form. When washed and plated in fresh plasma they put out processes and proliferate. After growth in the new plates has occurred the digestion and plating can be repeated. The limits of the method have not yet been reached. We are at work on a number of the problems which it has opened up.

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References

    1. J Exp Med. 1914 Apr 1;19(4):398-405 - PubMed
    1. J Exp Med. 1916 Feb 1;23(2):219-37 - PubMed