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. 1918 Sep 1;28(3):345-57.
doi: 10.1084/jem.28.3.345.

THE OPTIMUM HYDROGEN ION CONCENTRATION FOR THE GROWTH OF PNEUMOCOCCUS

K G Dernby  1 , O T Avery
Affiliations

THE OPTIMUM HYDROGEN ION CONCENTRATION FOR THE GROWTH OF PNEUMOCOCCUS

K G Dernby et al. J Exp Med. .

Abstract

1. The optimum hydrogen ion concentration for the growth of the various types of pneumococcus is a pH of about 7.8. 2. The limiting hydrogen ion concentrations for the growth of pneumococcus are a pH of 7.0 and a pH of 8.3. 3. Phosphates used in adjusting reactions of media retard growth if present in a concentration greater than 0.1 molecular. 4. Culture media for pneumococci should, therefore, have an initial reaction between a pH of 7.8 and 8.0 and a total salt concentration not exceeding 0.1 M.

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References

    1. Biochem J. 1911;5(5):207-14 - PubMed
    1. Biochem J. 1916 Oct;10(3):399-407 - PubMed